Science Behind Gene Insertion

Gene insertion, also known as targeted integration, is a type of gene editing that involves adding a new gene to a specific location in the genome. This is achieved through the use of nucleases, enzymes that cut DNA at specific locations. The cut DNA is then repaired using a DNA template that contains the desired gene. This results in the insertion of the new gene into the genome.

Gene insertion has the potential to revolutionize research with strains. Rather than correcting individual mutations, gene insertion can theoretically correct virtually all mutations in a gene. As a result, it is a highly versatile and powerful tool for developing new drugs based on the genetic component.

Fig. 1. cDNA insertion into the plasmid and the transformation of the E-coli the general steps. (Saifuddin N.M, 2001)

Services for Gene Insertion

CD Biosynsis is at the forefront of gene insertion technology, offering a range of services for gene insertion. The team of experts has extensive experience in the design and production of gene insertion vectors, as well as the use of nucleases for targeted integration.

Gene Insertion Vector Design

The design of an effective gene insertion vector is critical for the success of targeted integration. CD Biosynsis uses state-of-the-art software and algorithms to design vectors that are highly specific and efficient. Our team has experience designing vectors for a wide range of applications, including the correction of CFTR mutations in cystic fibrosis.

Nuclease-Mediated Gene Insertion

CD Biosynsis uses nucleases such as CRISPR/Cas9 and TALENs to achieve targeted integration. Our team has extensive experience in the use of these nucleases and can optimize their use for each individual application.

Ex Vivo Gene Insertion

CD Biosynsis offers ex vivo gene insertion services, which involve the modification of cells outside the body before they are transplanted back into the patient. This approach has been shown to be effective for the treatment of genetic disorders such as sickle cell anemia.

In Vivo Gene Insertion

CD Biosynsis is also developing in vivo gene insertion technologies, which involve the direct modification of cells within the body. This approach has the potential to be highly effective for the treatment of genetic disorders such as Huntington's disease.

Customized Solutions

We understand that each application is unique and requires a customized solution. That's why we work closely with our clients to understand their specific needs and develop customized solutions that meet their requirements.