CRISPR/Cas9 Off-target Validation Solution
https://www.igenetech.com/products/cas9-off-target-validation-solution/
#pcr #crispr
Overview
CRISPR off-target effect may result in anomalous insertions, deletions and translocation. It is critical to have a sensitive detection tool for the off-target editing. Based on the highly sensitive, accurate and stable TargetSeq® hybridization capture sequencing technology and MultipSeq® multiplex amplicon sequencing technology, iGeneTech provides a new, f... moreCRISPR/Cas9 Off-target Validation Solution
https://www.igenetech.com/products/cas9-off-target-validation-solution/
#pcr #crispr
Overview
CRISPR off-target effect may result in anomalous insertions, deletions and translocation. It is critical to have a sensitive detection tool for the off-target editing. Based on the highly sensitive, accurate and stable TargetSeq® hybridization capture sequencing technology and MultipSeq® multiplex amplicon sequencing technology, iGeneTech provides a new, fast and cost-effective solution for CRISPR off-target validation to detect hundreds of sites in one reaction.
Advantages
Professional and fast panel customized solution
Proven target sequencing technologies with excellent performance and simple workflow
Mutation detection limit as low as 1%
Identification of off-target sites is fast, accurate and cost intensive.
Cases
Factors determining editing efficiency and off-target cleavage activity for sgRNA
Researchers performed a study to understand the poorly documented factors that determine the editing efficiency and off-target cleavage activity of sgRNA by comparing the in-silico prediction and sequencing results from customized TargetSeq® panel. The result suggested that sgRNA activity and specificity can be sensitively detected by TargetSeq in combination with in-silico prediction.
