Microbial transglutaminase catalyzes acyl transfer reactions between the γ-carboxyamide group of a protein-bound glutamine residue and primary amines. In simple terms, microbial transglutaminase acts as a biological glue that covalently links proteins. Among others microbial transglutaminase is used in the food industry for the production of dairy products, meat, sausages and bakery products.
The Microbial Transglutaminase (MTG) ELISA (Product Nr. E021) is a sandwich enzyme immunoassay developed for the quantitative determination of native MTG. As part of the kit development, the following foods were examined: glued meat (raw), yoghurt, quark, cheese, salami and bread. It can be assumed that the test is also suitable for the analysis of further foods. Detection and determination limits depend on the respective sample matrix, the degree of processing and the extraction process.
Please note that for each type of sample material, the preparation procedure must be validated separately by the user.
All reagents for the implementation of the enzyme immunoassay are contained in the test kit. A test kit is sufficient for 96 determinations (including standards). A microtiter plate photometer is required for evaluation.
Sample preparation: homogenizing, extracting
Standard material: Microbial Transglutaminase (0 - 150 ng/mL)
Detection limit: 0.6 ng/mL (ppb) MTG (1 mL ≙ 1 g)
Determination limit: 3.0 ng/mL (ppb) MTG (1 mL ≙ 1 g)